SRR053857

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing (454 GS FLX) from unidentified mixed DNA library. Grade F; Q30 44.8% is critically low even by 454 standards, and duplication 96.7% suggests over-clustering. Small read count (23k reads) and low quality limit taxonomic conclusions. Reuse not recommended.

Data type / assay

amplicon

Organism

unidentified;mixed DNA library

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 12085084 reported

total reads 23165 reported

n content pct 0 measured

pct q20 bases 82.4 measured

pct q30 bases 44.8 measured

gc content pct 55.3 measured

mean read length 418.2 measured

mean base quality 27.1 measured

adapter content pct 0 measured

duplication rate pct 96.7 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 44.8 measured ×1 0%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0