SRR057512

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

26/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq of human tissue from legacy short-read platform. Grade F with score 26/100—poor quality, unsuitable for reuse. Q30 (48.7%), mean base quality (23.9), and extreme duplication (49.5%) all underperform; the combination of low sequence accuracy and severe PCR cloning will distort expression estimates and inflate false discovery rate.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 670274352 reported

total reads 18618732 reported

n content pct 0.757 measured

pct q20 bases 73.4 measured

pct q30 bases 48.7 measured

gc content pct 49.4 measured

mean read length 36 measured

mean base quality 23.9 measured

adapter content pct 0 measured

duplication rate pct 49.51 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 26/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 48.7 measured ×1 0%

mean base quality 23.9 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 49.51 measured ×0.4 57%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0