SRR059755

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

19/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from ant (Camponotus floridanus). Grade F; Q30 61.4% fails quality thresholds, compounded by high duplication (69.56%) suggesting aggressive PCR amplification. Despite large total bases, short mean read length (35 bp) and poor quality metrics make downstream assembly/mapping unreliable. Not recommended for reuse without heavy preprocessing.

Data type / assay

bulk-RNA-seq

Organism

Camponotus floridanus

Instrument

Illumina Genome Analyzer

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 626070515 reported

total reads 17887729 reported

n content pct 0.021 measured

pct q20 bases 85.1 measured

pct q30 bases 61.4 measured

gc content pct 47.2 measured

mean read length 35 measured

mean base quality 27.1 measured

adapter content pct 0 measured

duplication rate pct 69.56 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 19/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 61.4 measured ×1 0%

mean base quality 27.1 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 69.56 measured ×0.4 12%

QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0