SRR059760

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

19/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq of ant transcriptome from early-generation sequencer. Grade F with score 19/100—poor quality, avoid reuse. Low Q30 (54.9%), low base quality (25.3), and extreme duplication (70%) combine to produce unreliable bases and severely degraded effective read depth, making it unsuitable for any expression quantification study.

Data type / assay

bulk-RNA-seq

Organism

Harpegnathos saltator

Instrument

Illumina Genome Analyzer

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 516050920 reported

total reads 14744312 reported

n content pct 0.089 measured

pct q20 bases 79.4 measured

pct q30 bases 54.9 measured

gc content pct 47.5 measured

mean read length 35 measured

mean base quality 25.3 measured

adapter content pct 0 measured

duplication rate pct 70.01 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 19/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 54.9 measured ×1 0%

mean base quality 25.3 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 70.01 measured ×0.4 11%

QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0