SRR060663

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

26/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Drosophila virilis. Grade F; failed both Q30 (61.4%) and duplication (75.14%) thresholds, indicating degraded library and PCR over-cycling on Genome Analyzer II. High duplication artificially inflates read count without adding new molecules. Despite decent mean quality 30.3, not recommended for expression analysis.

Data type / assay

bulk-RNA-seq

Organism

Drosophila virilis

Instrument

Illumina Genome Analyzer

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 216376236 reported

total reads 6010451 reported

n content pct 0.201 measured

pct q20 bases 76 measured

pct q30 bases 61.4 measured

gc content pct 48.7 measured

mean read length 36 measured

mean base quality 30.3 measured

adapter content pct 0 measured

duplication rate pct 75.14 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 26/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 61.4 measured ×1 0%

mean base quality 30.3 measured ×0.6 38%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 75.14 measured ×0.4 0%

QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0