SRR070422

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

31/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Drosophila melanogaster EST (80bp short-read) with poor base quality (Grade F, 31/100). Q30 at 67.3% and mean quality of 24.9 are both failing metrics well below modern standards, indicating systematic low-fidelity sequencing; these defects will produce unreliable SNP calls and abundance estimates. Not recommended for reuse.

Data type / assay

bulk-RNA-seq

Organism

Drosophila melanogaster

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 6811048000 reported

total reads 42569050 reported

n content pct 0.947 measured

pct q20 bases 73.3 measured

pct q30 bases 67.3 measured

gc content pct 51.3 measured

mean read length 80 measured

mean base quality 24.9 measured

adapter content pct 0 measured

duplication rate pct 37.53 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 31/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 67.3 measured ×1 0%

mean base quality 24.9 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 37.53 measured ×0.4 83%

QC cost 37 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0