SRR070431

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

73/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read EST (gene-tag sequencing) from Drosophila melanogaster. Grade C; mid-tier quality with Q30 85.1% and mean base quality 29.9, acceptable for EST catalogs but slightly elevated Q20 thresholds. Unusual 1.176% N-content (atypical for Illumina) suggests library prep variability. Long mean read length (80 bp) partially compensates; suitable for EST work with caveats.

Data type / assay

bulk-RNA-seq

Organism

Drosophila melanogaster

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3639435360 reported

total reads 22746471 reported

n content pct 1.176 measured

pct q20 bases 88.2 measured

pct q30 bases 85.1 measured

gc content pct 52.1 measured

mean read length 80 measured

mean base quality 29.9 measured

adapter content pct 0 measured

duplication rate pct 25.7 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 73/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.1 measured ×1 76%

mean base quality 29.9 measured ×0.6 32%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 25.7 measured ×0.4 100%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0