SRR10034570

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Apis cerana miRNA-seq with severe library-preparation artifacts (Grade C, 71/100). Adapter contamination at 19% and extreme duplication at 66.89% are the twin drivers of low quality, indicating incomplete adapter removal and runaway PCR amplification; absolute abundance estimates will be severely biased. Not suitable for quantitative miRNA studies.

Data type / assay

bulk-RNA-seq

Organism

Apis cerana

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 663196500 reported

total reads 13263930 reported

n content pct 0.004 measured

pct q20 bases 97.1 measured

pct q30 bases 93.6 measured

gc content pct 51.1 measured

mean read length 50 measured

mean base quality 35.4 measured

adapter content pct 19 measured

duplication rate pct 66.89 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.6 measured ×1 100%

mean base quality 35.4 measured ×0.6 100%

adapter content pct 19 measured ×0.4 6%

duplication rate pct 66.89 measured ×0.4 18%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0