SRR10035763

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bombyx mori bulk RNA-seq from NovaSeq platform in high-throughput condition (Grade A, 96/100). Elevated duplication at 41.98% is the primary limiting metric and indicates moderate PCR bias that will skew expression quantification; offset by excellent base quality (Q30=94.7%, mean=36.1). Suitable for reuse with duplication-aware normalization.

Data type / assay

bulk-RNA-seq

Organism

Bombyx mori

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7508608800 reported

total reads 25028696 reported

n content pct 0 measured

pct q20 bases 98.2 measured

pct q30 bases 94.7 measured

gc content pct 44.2 measured

mean read length 150 measured

mean base quality 36.1 measured

adapter content pct 0 measured

duplication rate pct 41.98 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.7 measured ×1 100%

mean base quality 36.1 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 41.98 measured ×0.4 73%

QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0