SRR10079135

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from Tribolium castaneum with good base quality (97.3% Q30) but extremely high sequence duplication at 79.3%, severely limiting transcript abundance estimates and expression analysis reliability; investigate whether this reflects genuine biology or library preparation artifacts.

Data type / assay

bulk-RNA-seq

Organism

Tribolium castaneum

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1176283704 reported

total reads 16479740 reported

n content pct 0.005 measured

pct q20 bases 98.1 measured

pct q30 bases 97.3 measured

gc content pct 36.1 measured

mean read length 71.4 measured

mean base quality 35.1 measured

adapter content pct 0 measured

duplication rate pct 79.3 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.3 measured ×1 100%

mean base quality 35.1 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 79.3 measured ×0.4 0%

QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0