SRR10125242

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

92/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk short-read RNA-seq with exceptional basecall quality (Q30=95.9%, mean Q=36.0) but higher technical duplication at 51.97% (scored 51/100, weight 0.4) limits quantification accuracy. Long reads (124.7 bp) and large depth (4.8 Gb) support robust transcript discovery. Suitable for reuse where moderate PCR bias can be accommodated.

Data type / assay

bulk-RNA-seq

Organism

Contarinia nasturtii

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4777484530 reported

total reads 19176948 reported

n content pct 0 measured

pct q20 bases 98.2 measured

pct q30 bases 95.9 measured

gc content pct 41.2 measured

mean read length 124.7 measured

mean base quality 36 measured

adapter content pct 0.01 measured

duplication rate pct 51.97 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 92/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.9 measured ×1 100%

mean base quality 36 measured ×0.6 100%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 51.97 measured ×0.4 51%

QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0