SRR10158872

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

92/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk short-read RNA-seq from a modest-size run (1.49M reads, 93 Mb) with good basecall quality (mean Q=35.2) but Q30 at 86.3% represents the primary constraint (scored 82/100, weight 1.0). Low duplication (24.06%) and minimal adapters ensure clean library prep. Suitable for reuse pending independent verification of sample adequacy for downstream analyses.

Data type / assay

bulk-RNA-seq

Organism

Aphis glycines

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 93321303 reported

total reads 1493660 reported

n content pct 0.01 measured

pct q20 bases 96.9 measured

pct q30 bases 86.3 measured

gc content pct 64.2 measured

mean read length 61.7 measured

mean base quality 35.2 measured

adapter content pct 0.06 measured

duplication rate pct 24.06 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 92/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 86.3 measured ×1 82%

mean base quality 35.2 measured ×0.6 100%

adapter content pct 0.06 measured ×0.4 100%

duplication rate pct 24.06 measured ×0.4 100%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0