SRR10158881

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

84/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Aphis glycines RNA-seq from HiSeq 2000 generating ~5.5M reads with moderate quality (Q30: 82.2%, Q20: 94.6%), suitable for relative expression work but lower confidence for SNV detection. The small library size limits power for low-abundance transcript discovery.

Data type / assay

bulk-RNA-seq

Organism

Aphis glycines

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 406127642 reported

total reads 5508538 reported

n content pct 0 measured

pct q20 bases 94.6 measured

pct q30 bases 82.2 measured

gc content pct 42 measured

mean read length 68.4 measured

mean base quality 34.2 measured

adapter content pct 0.09 measured

duplication rate pct 18.26 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 84/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.2 measured ×1 61%

mean base quality 34.2 measured ×0.6 100%

adapter content pct 0.09 measured ×0.4 100%

duplication rate pct 18.26 measured ×0.4 100%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0