SRR10158886

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

70/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Moderate-quality 454 pyrosequencing RNA-seq with inherently lower Q30 coverage (75.6%) typical of the platform, offset by long read length (355.9bp) and low duplication (8.29%); usable for transcript assembly and novel isoform discovery but error rates require careful post-processing and filtering to avoid spurious variants in downstream analysis.

Data type / assay

bulk-RNA-seq

Organism

Aphis glycines

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 599806774 reported

total reads 1671051 reported

n content pct 0.017 measured

pct q20 bases 90.3 measured

pct q30 bases 75.6 measured

gc content pct 33.5 measured

mean read length 355.9 measured

mean base quality 33.9 measured

adapter content pct 0 measured

duplication rate pct 8.29 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 70/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.6 measured ×1 28%

mean base quality 33.9 measured ×0.6 98%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 8.29 measured ×0.4 100%

QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0