SRR10231810

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

44/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

ncRNA-seq with catastrophic technical failure: 95.35% adapter content (scored 0/100) indicates library construction breakdown, and 75.85% duplication (scored 0/100) reflects extreme amplification bias. Dataset is predominantly technical artifacts with minimal usable reads. Not recommended for reuse without re-sequencing.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 576522525 reported

total reads 7686967 reported

n content pct 0.001 measured

pct q20 bases 88.1 measured

pct q30 bases 82.9 measured

gc content pct 48.8 measured

mean read length 75 measured

mean base quality 32.1 measured

adapter content pct 95.35 measured

duplication rate pct 75.85 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 44/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.9 measured ×1 65%

mean base quality 32.1 measured ×0.6 68%

adapter content pct 95.35 measured ×0.4 0%

duplication rate pct 75.85 measured ×0.4 0%

QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0