SRR10231851

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk short-read RNA-seq with paradoxical profile: excellent basecall quality (Q30=95.2%) contrasted against devastating technical duplication at 80.9% (scored 0/100, weight 0.4), which dominates the B-grade (83/100). High-quality bases cannot offset severe PCR bias rendering abundance estimates unreliable. Marginal for reuse; would require aggressive normalization.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 975207300 reported

total reads 6501382 reported

n content pct 0.007 measured

pct q20 bases 96.7 measured

pct q30 bases 95.2 measured

gc content pct 39.1 measured

mean read length 75 measured

mean base quality 34.6 measured

adapter content pct 0 measured

duplication rate pct 80.9 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.2 measured ×1 100%

mean base quality 34.6 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 80.9 measured ×0.4 0%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0