SRR1025185

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

18/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Severely degraded miRNA-seq with critical base-quality failure—only 60.7% Q30 bases, mean Q=27.5, and extreme duplication (70.2%)—rendering it unsuitable for reliable miRNA quantification or discovery; low read length (35bp) and high error rate compound the difficulty. This dataset should not be used without major resequencing.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 316791265 reported

total reads 9051179 reported

n content pct 0.022 measured

pct q20 bases 86.4 measured

pct q30 bases 60.7 measured

gc content pct 50.7 measured

mean read length 35 measured

mean base quality 27.5 measured

adapter content pct 0 measured

duplication rate pct 70.2 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 18/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 60.7 measured ×1 0%

mean base quality 27.5 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 70.2 measured ×0.4 11%

QC cost 37 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0