SRR1025186

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

17/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina miRNA-seq; grade F, unsuitable for reuse. Poor base quality (Q30=61.7%, mean BQ=27.7) combined with high duplication (73.5%) critically undermine miRNA detection specificity and expression estimate reliability.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 321779710 reported

total reads 9193706 reported

n content pct 0.017 measured

pct q20 bases 87.2 measured

pct q30 bases 61.7 measured

gc content pct 50.5 measured

mean read length 35 measured

mean base quality 27.7 measured

adapter content pct 0 measured

duplication rate pct 73.5 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 17/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 61.7 measured ×1 0%

mean base quality 27.7 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 73.5 measured ×0.4 3%

QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0