SRR10291791

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

75/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk short-read RNA-seq with severe technical duplication at 83.54% (scored 0/100, weight 0.4), which dominates and renders abundance estimates unreliable despite reasonable Q30 of 88%. Extreme PCR bias is the primary disqualifier. Marginal for relative expression with strong normalization; absolute quantification not recommended.

Data type / assay

bulk-RNA-seq

Organism

Aphis gossypii

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1336037111 reported

total reads 18203591 reported

n content pct 0.001 measured

pct q20 bases 92.2 measured

pct q30 bases 88 measured

gc content pct 62.6 measured

mean read length 73.3 measured

mean base quality 33.1 measured

adapter content pct 0.03 measured

duplication rate pct 83.54 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 75/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88 measured ×1 90%

mean base quality 33.1 measured ×0.6 85%

adapter content pct 0.03 measured ×0.4 100%

duplication rate pct 83.54 measured ×0.4 0%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0