SRR10291792

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina short-read bulk RNA-seq; grade C, conditional/marginal reuse. Excellent base quality (Q30=88.1%) is negated by extreme duplication (84.48%), which systematically inflates counts for abundant transcripts and masks lowly-expressed genes.

Data type / assay

bulk-RNA-seq

Organism

Aphis gossypii

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1592793286 reported

total reads 21668065 reported

n content pct 0.001 measured

pct q20 bases 92.3 measured

pct q30 bases 88.1 measured

gc content pct 63.5 measured

mean read length 73.5 measured

mean base quality 33.2 measured

adapter content pct 0.03 measured

duplication rate pct 84.48 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.1 measured ×1 91%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 0.03 measured ×0.4 100%

duplication rate pct 84.48 measured ×0.4 0%

QC cost 42 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0