SRR1048130

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

84/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

101bp short-read bulk RNA-seq from Tribolium castaneum at acceptable quality (grade B); reuse requires downstream quality filtering. Measured Q30 bases at 84.1% (scored 70/100) and mean base quality 33.2 are the key limitations; elevated measured N content (0.245%) and adapter content (1.3%) suggest incomplete preprocessing, demanding extra care during alignment.

Data type / assay

bulk-RNA-seq

Organism

Tribolium castaneum

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5530045728 reported

total reads 27376464 reported

n content pct 0.245 measured

pct q20 bases 90.6 measured

pct q30 bases 84.1 measured

gc content pct 44.5 measured

mean read length 101 measured

mean base quality 33.2 measured

adapter content pct 1.3 measured

duplication rate pct 25.25 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 84/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.1 measured ×1 71%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 1.3 measured ×0.4 100%

duplication rate pct 25.25 measured ×0.4 100%

QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0