SRR1048134

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

73/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Tribolium castaneum RNA-seq on HiSeq 2000 yielding 6.3 billion bases (88.4% Q20), slightly reduced quality compared to sibling samples but sufficient for transcript quantitation; high read count compensates for 80.6% Q30 rate, supporting statistical power for differential expression studies.

Data type / assay

bulk-RNA-seq

Organism

Tribolium castaneum

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 6313499092 reported

total reads 31254946 reported

n content pct 0.004 measured

pct q20 bases 88.4 measured

pct q30 bases 80.6 measured

gc content pct 44.1 measured

mean read length 101 measured

mean base quality 32.2 measured

adapter content pct 0.63 measured

duplication rate pct 27.41 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 73/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.6 measured ×1 53%

mean base quality 32.2 measured ×0.6 70%

adapter content pct 0.63 measured ×0.4 100%

duplication rate pct 27.41 measured ×0.4 100%

QC cost 38 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0