Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
← Dataset search

SRR10489571

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Short-read bulk RNA-seq from Anopheles gambiae at good quality (grade A). Measured Q30 at 94.5% remains excellent for basecall accuracy; however, measured duplication at 41.62% (scored 74/100) is the primary concern, indicating substantial PCR bias that may distort expression estimates for abundant transcripts.

Data type / assay
bulk-RNA-seq
Organism
Anopheles gambiae
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4886547749 reported
total reads 32335226 reported
n content pct 0.006 measured
pct q20 bases 96.5 measured
pct q30 bases 94.5 measured
gc content pct 48.2 measured
mean read length 75.6 measured
mean base quality 34.5 measured
adapter content pct 0 measured
duplication rate pct 41.62 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.5 measured ×1 100%
mean base quality 34.5 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 41.62 measured ×0.4 74%
QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0