Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR1055985

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

60/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This HiSeq 2000 RNA-seq from the fungus Neurospora crassa produced 42.9M reads across 2.1B bases with notably lower Q30 (76.9%) and elevated N-content (0.186%), indicating degraded sample or library quality. The 55.9% GC is high for fungi. Reuse requires understanding whether quality issues reflect biological sample degradation or technical failure; caveat that lower accuracy compromises isoform-level quantitation and variant calling.

Data type / assay
bulk-RNA-seq
Organism
Neurospora crassa
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2190139308 reported
total reads 42943908 reported
n content pct 0.186 measured
pct q20 bases 83.9 measured
pct q30 bases 76.9 measured
gc content pct 55.9 measured
mean read length 51 measured
mean base quality 31.1 measured
adapter content pct 0.11 measured
duplication rate pct 31.38 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 60/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76.9 measured ×1 35%
mean base quality 31.1 measured ×0.6 52%
adapter content pct 0.11 measured ×0.4 100%
duplication rate pct 31.38 measured ×0.4 97%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0