Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR1060997

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Genome Analyzer IIx RNA-seq from Aedes aegypti generated 1.4M reads spanning 148M bases with very low Q20 (89.4%) and Q30 (83.2%), plus markedly elevated N-content (0.488%), indicating severely compromised data quality. The 44.8% GC is normal but overall data integrity is questionable. Reuse is not recommended; this dataset reflects likely sample degradation or platform malfunction and cannot support reliable expression analysis.

Data type / assay
bulk-RNA-seq
Organism
Aedes aegypti
Instrument
Illumina Genome Analyzer IIx
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 148952300 reported
total reads 1489523 reported
n content pct 0.488 measured
pct q20 bases 89.4 measured
pct q30 bases 83.2 measured
gc content pct 44.8 measured
mean read length 50 measured
mean base quality 33.7 measured
adapter content pct 0.19 measured
duplication rate pct 27.29 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.2 measured ×1 66%
mean base quality 33.7 measured ×0.6 95%
adapter content pct 0.19 measured ×0.4 100%
duplication rate pct 27.29 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0