Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR1103958

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

60/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Illumina HiSeq 2000 bulk RNA-seq from Aedes aegypti comprises 82.1 million short reads totaling 8.8 billion bases at moderate quality (82.6% ≥Q20, 76.9% ≥Q30). The notably high N content (2.443%) is markedly unusual and indicates significant quality issues with this run. The high read count partially compensates, but the elevated N content requires conservative downstream filtering and limits utility for sensitive gene expression analysis.

Data type / assay
bulk-RNA-seq
Organism
Aedes aegypti
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 8786494390 reported
total reads 82116770 reported
n content pct 2.443 measured
pct q20 bases 82.6 measured
pct q30 bases 76.9 measured
gc content pct 44.9 measured
mean read length 107 measured
mean base quality 31 measured
adapter content pct 0.01 measured
duplication rate pct 13.47 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 60/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76.9 measured ×1 35%
mean base quality 31 measured ×0.6 50%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 13.47 measured ×0.4 100%
QC cost 9 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0