Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR11428781

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

35/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NextSeq 550 bulk RNA-seq from Hormaphis cornu yields ~15.6 million short reads (4.69 billion bases, 75% Q30), moderate coverage with notably lower Q30 and Q20 (80.5%), requiring quality consideration. This dataset supports transcript quantification with careful filtering; differential expression studies require attention to sequence quality. Bulk sequencing lacks cell-type specificity; reuse suits focused transcriptomic studies of aphid gene expression.

Data type / assay
bulk-RNA-seq
Organism
Hormaphis cornu
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4685495400 reported
total reads 15618318 reported
n content pct 0.001 measured
pct q20 bases 80.5 measured
pct q30 bases 75 measured
gc content pct 37.8 measured
mean read length 150 measured
mean base quality 30.5 measured
adapter content pct 18.81 measured
duplication rate pct 39.62 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 35/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75 measured ×1 25%
mean base quality 30.5 measured ×0.6 42%
adapter content pct 18.81 measured ×0.4 7%
duplication rate pct 39.62 measured ×0.4 79%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0