Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR1166498

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

HiSeq 2000 bulk RNA-seq from Aedes aegypti produces ~94.2 million short reads (9.51 billion bases, 79% Q30), very high coverage with elevated N-content (0.102%) and notably lower quality requiring significant filtering. This massive dataset supports comprehensive transcriptome profiling after quality control; differential expression studies require careful error handling. Bulk sequencing lacks cell-type specificity; reuse suits large-scale comparative transcriptomic studies of Aedes.

Data type / assay
bulk-RNA-seq
Organism
Aedes aegypti
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 9510882251 reported
total reads 94167151 reported
n content pct 0.102 measured
pct q20 bases 87.3 measured
pct q30 bases 79 measured
gc content pct 44.9 measured
mean read length 101 measured
mean base quality 31.8 measured
adapter content pct 2.21 measured
duplication rate pct 16.09 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 79 measured ×1 45%
mean base quality 31.8 measured ×0.6 63%
adapter content pct 2.21 measured ×0.4 99%
duplication rate pct 16.09 measured ×0.4 100%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0