SRR1174029

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Culicoides sonorensis (biting midge) RNA-seq from HiSeq 2000 with 42M reads and exceptional quality (99% Q20, 94.3% Q30). Outstanding basecall accuracy—among the highest in the dataset—supports high-confidence isoform and variant analysis in this understudied Culicoides vector. Minimal N (0.001%) ensures clean sequence data for expression or assembly-based studies.

Data type / assay

bulk-RNA-seq

Organism

Culicoides sonorensis

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8200938961 reported

total reads 41567782 reported

n content pct 0.001 measured

pct q20 bases 99 measured

pct q30 bases 94.3 measured

gc content pct 36.9 measured

mean read length 98.4 measured

mean base quality 36.7 measured

adapter content pct 0.96 measured

duplication rate pct 40.27 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%

mean base quality 36.7 measured ×0.6 100%

adapter content pct 0.96 measured ×0.4 100%

duplication rate pct 40.27 measured ×0.4 77%

QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0