SRR11819624

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

62/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-Seq from beetle (Illumina HiSeq 4000). Grade D/62—the worst performer in this cohort with 86.42% duplication (scored 0/100, weight 0.4), mean base quality of only 30 (scored 33/100, weight 0.6), and very short reads (35.4bp). These compounded defects render this dataset unsuitable for reliable expression analysis.

Data type / assay

bulk-RNA-seq

Organism

Tribolium castaneum

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 683698692 reported

total reads 19121057 reported

n content pct 0 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 51.8 measured

mean read length 35.4 measured

mean base quality 30 measured

adapter content pct 6.82 measured

duplication rate pct 86.42 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 62/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 6.82 measured ×0.4 73%

duplication rate pct 86.42 measured ×0.4 0%

QC cost 39 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0