Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR12008220

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This HiSeq 4000 RNA-seq from the brown planthopper Nilaparvata lugens generated 28.3M reads spanning 8.4B bases with 96.3% Q20 and 91.2% Q30, providing substantial transcriptome depth for studying insect-plant interactions and pest-resistance mechanisms. The 46.5% GC is expected. Reuse is ideal for identifying host-recognition and detoxification genes; caveat that diet and pesticide exposure strongly modulate transcriptional responses.

Data type / assay
bulk-RNA-seq
Organism
Nilaparvata lugens
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 8492790900 reported
total reads 28309303 reported
n content pct 0.004 measured
pct q20 bases 96.3 measured
pct q30 bases 91.2 measured
gc content pct 46.5 measured
mean read length 150 measured
mean base quality 38 measured
adapter content pct 0.63 measured
duplication rate pct 27.58 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.2 measured ×1 100%
mean base quality 38 measured ×0.6 100%
adapter content pct 0.63 measured ×0.4 100%
duplication rate pct 27.58 measured ×0.4 100%
QC cost 40 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0