SRR12180676

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Locusta migratoria (desert locust) RNA-seq from NovaSeq 6000 with 20M reads and outstanding quality (97.7% Q20, 93.4% Q30). Modern high-throughput platform delivers clean data despite modest read count; high basecall quality compensates and enables sensitive transcript-level analysis in this pest-relevant orthopteran species. Balanced GC (48%) and zero N support transcript discovery.

Data type / assay

bulk-RNA-seq

Organism

Locusta migratoria

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5938256100 reported

total reads 19794187 reported

n content pct 0 measured

pct q20 bases 97.7 measured

pct q30 bases 93.4 measured

gc content pct 48 measured

mean read length 150 measured

mean base quality 35.9 measured

adapter content pct 1.22 measured

duplication rate pct 42.73 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.4 measured ×1 100%

mean base quality 35.9 measured ×0.6 100%

adapter content pct 1.22 measured ×0.4 100%

duplication rate pct 42.73 measured ×0.4 72%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0