Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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SRR12351707

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

77/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

SARS-CoV-2 amplicon (Illumina NovaSeq 6000). Grade C/77—96.25% duplication is extreme and dominant, rendering nearly all reads non-independent PCR replicates. Longer reads (249bp mean) offer some utility, but duplication burden is prohibitive; Q30 (83.2%, scored 66/100, weight 1) is lower than ideal, and these combine to severely limit variant detection confidence.

Data type / assay
amplicon
Organism
Severe acute respiratory syndrome coronavirus 2
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1989486594 reported
total reads 3994953 reported
n content pct 0.002 measured
pct q20 bases 93 measured
pct q30 bases 83.2 measured
gc content pct 39.7 measured
mean read length 249 measured
mean base quality 34 measured
adapter content pct 0.99 measured
duplication rate pct 96.25 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 77/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.2 measured ×1 66%
adapter content pct 0.99 measured ×0.5 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0