SRR12358269

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Helicoverpa bulk RNA-seq; excellent measured Q30 (97.8%) and base quality (QV=39.9) are offset by duplication rate of 40.3%, which scored 77/100 and limits overall grade to 96/100. Duplication is expected in RNA-seq but at the higher end; carefully filter technical duplicates during expression quantification to avoid inflating low-abundance transcript estimates.

Data type / assay

bulk-RNA-seq

Organism

Helicoverpa armigera

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1415627312 reported

total reads 7008056 reported

n content pct 0.005 measured

pct q20 bases 99.3 measured

pct q30 bases 97.8 measured

gc content pct 47.9 measured

mean read length 101 measured

mean base quality 39.9 measured

adapter content pct 0.38 measured

duplication rate pct 40.32 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.8 measured ×1 100%

mean base quality 39.9 measured ×0.6 100%

adapter content pct 0.38 measured ×0.4 100%

duplication rate pct 40.32 measured ×0.4 77%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0