SRR12362619

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Lutzomyia longipalpis bulk RNA-Seq from HiSeq 2500 with 26M reads at consistent quality (96.3% ≥Q20, 93% ≥Q30). Enables transcriptomic characterization of sandfly gene expression, development, and vector competence for leishmaniasis transmission.

Data type / assay

bulk-RNA-seq

Organism

Lutzomyia longipalpis

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3330978375 reported

total reads 26647827 reported

n content pct 0.032 measured

pct q20 bases 96.3 measured

pct q30 bases 93 measured

gc content pct 41.5 measured

mean read length 125 measured

mean base quality 36.2 measured

adapter content pct 1.2 measured

duplication rate pct 36.23 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93 measured ×1 100%

mean base quality 36.2 measured ×0.6 100%

adapter content pct 1.2 measured ×0.4 100%

duplication rate pct 36.23 measured ×0.4 86%

QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0