SRR12376916

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-Seq from beetle (Illumina HiSeq 2500). Grade A/100—excellent base quality (94% Q30, 37.8 mean BQ) combined with minimal adapter (2.25%, scored 99/100) and low duplication (27.51%) across 150bp reads. Strong metrics across all dimensions support reliable comprehensive transcriptomic profiling.

Data type / assay

bulk-RNA-seq

Organism

Tribolium castaneum

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7792190100 reported

total reads 25973967 reported

n content pct 0.001 measured

pct q20 bases 97.7 measured

pct q30 bases 94 measured

gc content pct 45.7 measured

mean read length 150 measured

mean base quality 37.8 measured

adapter content pct 2.25 measured

duplication rate pct 27.51 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94 measured ×1 100%

mean base quality 37.8 measured ×0.6 100%

adapter content pct 2.25 measured ×0.4 99%

duplication rate pct 27.51 measured ×0.4 100%

QC cost 52 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0