SRR12384461

ENA first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Targeted amplicon sequencing of Escherichia coli from a NovaSeq 6000 with ~348k short reads and high quality (96.4% Q30), suggesting focused interrogation of genomic regions such as antibiotic resistance genes or virulence markers. Appropriate for species identification, strain typing, or locus-specific abundance estimation in bacterial surveillance.

Data type / assay

amplicon

Organism

Escherichia coli

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 101487520 reported

total reads 347560 reported

n content pct 0.002 measured

pct q20 bases 98.9 measured

pct q30 bases 96.4 measured

gc content pct 47.7 measured

mean read length 146 measured

mean base quality 36.4 measured

adapter content pct 0 measured

duplication rate pct 99.7 measured

mean target coverage 1103.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.4 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0