Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR12695278

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Danaus plexippus (monarch butterfly) bulk RNA-seq on a NextSeq 500 comprises 19.1 million short reads (2.9 billion bases) with notably lower quality metrics (87.5% Q20, 82.9% Q30) than typical RNA-seq standards. The elevated GC content (48.4%) may indicate biased amplification or tissue-specific composition. Usable for initial expression screening, but the lower Q30 requires aggressive trimming; verify sample integrity before committing to quantitative cross-sample comparisons.

Data type / assay
bulk-RNA-seq
Organism
Danaus plexippus
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2895598731 reported
total reads 19158061 reported
n content pct 0.001 measured
pct q20 bases 87.5 measured
pct q30 bases 82.9 measured
gc content pct 48.4 measured
mean read length 75.6 measured
mean base quality 32.1 measured
adapter content pct 0 measured
duplication rate pct 45.97 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.9 measured ×1 65%
mean base quality 32.1 measured ×0.6 68%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 45.97 measured ×0.4 65%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0