SRR12695280

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Large-scale monarch butterfly (Danaus plexippus) bulk RNA-Seq dataset on HiSeq 2500 with substantial sequencing depth (40M reads, 8B bases). High base quality (98.4% ≥Q20, 95.1% ≥Q30) supports robust gene expression quantification and isoform analysis for lepidopteran developmental or ecological transcriptomics.

Data type / assay

bulk-RNA-seq

Organism

Danaus plexippus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8025832200 reported

total reads 40129161 reported

n content pct 0.012 measured

pct q20 bases 98.4 measured

pct q30 bases 95.1 measured

gc content pct 48.7 measured

mean read length 100 measured

mean base quality 39 measured

adapter content pct 0.46 measured

duplication rate pct 68.1 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.1 measured ×1 100%

mean base quality 39 measured ×0.6 100%

adapter content pct 0.46 measured ×0.4 100%

duplication rate pct 68.1 measured ×0.4 15%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0