SRR1269675

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

47/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

454 pyrosequencing bulk RNA with platform-inherent quality ceiling: Q30=63.8% (far below modern standards) and weak mean base quality (31.3%)—legacy technology unsuitable for reuse in current analysis pipelines. Evidence strength is low.

Data type / assay

bulk-RNA-seq

Organism

Cydia pomonella

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 85371485 reported

total reads 160324 reported

n content pct 0.035 measured

pct q20 bases 85 measured

pct q30 bases 63.8 measured

gc content pct 42.9 measured

mean read length 372.4 measured

mean base quality 31.3 measured

adapter content pct 0 measured

duplication rate pct 28.15 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 47/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 63.8 measured ×1 0%

mean base quality 31.3 measured ×0.6 55%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 28.15 measured ×0.4 100%

QC cost 47 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0