SRR1271735

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Culex bulk RNA-seq with strong base quality (Q30=93.5%), minimal adapter content (0.24%), and manageable duplication (34.15%)—exemplifies reusable RNA-seq with no significant QC liabilities. Grade A justifies confident reuse. Evidence strength is low.

Data type / assay

bulk-RNA-seq

Organism

Culex quinquefasciatus

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 577083682 reported

total reads 11777218 reported

n content pct 0.001 measured

pct q20 bases 97.8 measured

pct q30 bases 93.5 measured

gc content pct 44 measured

mean read length 49 measured

mean base quality 35.7 measured

adapter content pct 0.24 measured

duplication rate pct 34.15 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.5 measured ×1 100%

mean base quality 35.7 measured ×0.6 100%

adapter content pct 0.24 measured ×0.4 100%

duplication rate pct 34.15 measured ×0.4 91%

QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0