Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
← Dataset search

SRR12763477

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

SARS-CoV-2 amplicon sequencing with poor reusability. Grade D driven by adapter_content_pct=19.41 (measured), which scores 0/100 despite excellent base quality (Q30=98.8%). Residual primer/adapter sequences in amplicon data skew viral abundance estimates and complicate variant calling; requires aggressive trimming before reuse. Base quality alone does not salvage this run.

Data type / assay
amplicon
Organism
Severe acute respiratory syndrome coronavirus 2
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 29278548 reported
total reads 100269 reported
n content pct 0 measured
pct q20 bases 99.7 measured
pct q30 bases 98.8 measured
gc content pct 39.1 measured
mean read length 146 measured
mean base quality 37.5 measured
adapter content pct 19.41 measured
duplication rate pct 73.91 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.8 measured ×1 100%
adapter content pct 19.41 measured ×0.5 0%
QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0