SRR12808934

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 500 bulk RNA-Seq of Melitaea cinxia generated 5.75M reads (874 Mb) with high quality (94% Q30, 43.3% GC). This butterfly transcriptome provides gene expression data for understanding development and immune responses.

Data type / assay

bulk-RNA-seq

Organism

Melitaea cinxia

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 874403104 reported

total reads 5752652 reported

n content pct 0.01 measured

pct q20 bases 95.7 measured

pct q30 bases 94 measured

gc content pct 43.3 measured

mean read length 86 measured

mean base quality 34.4 measured

adapter content pct 0.18 measured

duplication rate pct 48.95 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94 measured ×1 100%

mean base quality 34.4 measured ×0.6 100%

adapter content pct 0.18 measured ×0.4 100%

duplication rate pct 48.95 measured ×0.4 58%

QC cost 57 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0