SRR12833577

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

SARS-CoV-2 amplicon sequencing on NovaSeq 6000 yielding ~2.9M reads with robust quality (Q30: 96.3%, Q20: 98.9%). This depth and accuracy enable sensitive detection of co-occurring mutations and population-level viral heterogeneity within the amplicon design.

Data type / assay

amplicon

Organism

Severe acute respiratory syndrome coronavirus 2

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 855678260 reported

total reads 2930405 reported

n content pct 0.001 measured

pct q20 bases 98.9 measured

pct q30 bases 96.3 measured

gc content pct 38.4 measured

mean read length 146 measured

mean base quality 36.4 measured

adapter content pct 12.13 measured

duplication rate pct 91.18 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.3 measured ×1 100%

adapter content pct 12.13 measured ×0.5 21%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0