Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
← Dataset search

SRR12833774

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

72/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Illumina NovaSeq 6000 amplicon sequencing run for SARS-CoV-2 produced 2.9 million short reads (875.4 million bases) with exceptional quality (98.7% Q20, 95.7% Q30), leveraging the NovaSeq platform's higher throughput than NextSeq instruments. Minimal N-content indicates pristine amplicon preparation. Higher read count supports deeper per-amplicon coverage and allows multiplexing of more samples simultaneously without sacrificing sequencing depth.

Data type / assay
amplicon
Organism
Severe acute respiratory syndrome coronavirus 2
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 875407240 reported
total reads 2997970 reported
n content pct 0.001 measured
pct q20 bases 98.7 measured
pct q30 bases 95.7 measured
gc content pct 38.4 measured
mean read length 146 measured
mean base quality 36.3 measured
adapter content pct 12.55 measured
duplication rate pct 89.63 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 72/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.7 measured ×1 100%
adapter content pct 12.55 measured ×0.5 18%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0