Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR1291267

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a human small-RNA (miRNA-Seq) library on the HiSeq 2000, and its B/83 grade reflects genuinely excellent base-level quality tempered by one expected anomaly. The grade was driven up by pristine base calling — pct_q30_bases and mean_base_quality both maxed out, with zero adapter and zero N content, meaning trimmed reads should map cleanly — and the ~22 nt mean read length is exactly right for mature miRNAs, so the assay looks on-target. The single metric pulling the score down is the 97.65% duplication rate, which scored 0; this would be alarming in mRNA-seq, but for miRNA-Seq it is largely an artifact of a small, naturally low-complexity target space where the same handful of abundant miRNA sequences recur, so it should not by itself disqualify reuse for differential-expression work. Because all contributing metrics were actually measured (evidence_strength = 1), this reading is solid rather than provisional, though you should still confirm library complexity and unique-miRNA yield before trusting it for novel-miRNA discovery.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 692253810 reported
total reads 31305708 reported
n content pct 0 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 38.8 measured
mean read length 22.1 measured
mean base quality 68.1 measured
adapter content pct 0 measured
duplication rate pct 97.65 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
mean base quality 68.1 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 97.65 measured ×0.4 0%
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89