SRR1297081

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

57/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

WGS (Escherichia coli), Grade F, evidence=0.61 (provisional). Q30 at 62.1% (scored 0) and extrapolated coverage (199.6x) are both problematic. Measured Q30 failure and provisional coverage estimate disqualify this for variant discovery; measured depth confirmation is mandatory.

Data type / assay

WGS

Organism

Escherichia coli

Instrument

AB 5500xl Genetic Analyzer

Platform

ABI_SOLID

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 918114700 reported

total reads 18362294 reported

mean coverage 199.6 extrapolated

n content pct 0 measured

pct q20 bases 83.1 measured

pct q30 bases 62.1 measured

gc content pct 49.8 measured

mean read length 50 measured

mean base quality 26.9 measured

adapter content pct 0 measured

duplication rate pct 29.3 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 57/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 199.6 extrapolated ×1.2 100%

pct q30 bases 62.1 measured ×1 0%

duplication rate pct 29.3 measured ×0.5 33%

adapter content pct 0 measured ×0.4 100%

QC cost 20 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0