SRR1297133

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

62/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

AB 5500xl (SOLiD) WGS of *Escherichia coli* achieves 12.8M reads with 82.7% Q20 and 58.8% Q30—significant platform-inherent quality limitations requiring elevated sequencing depth and permissive assembly parameters for genome reconstruction. Search: *E. coli* WGS, SOLiD platform, bacterial sequencing.

Data type / assay

WGS

Organism

Escherichia coli

Instrument

AB 5500xl Genetic Analyzer

Platform

ABI_SOLID

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 639592250 reported

total reads 12791845 reported

mean coverage 139 extrapolated

n content pct 0 measured

pct q20 bases 82.7 measured

pct q30 bases 58.8 measured

gc content pct 50 measured

mean read length 50 measured

mean base quality 26.8 measured

adapter content pct 0 measured

duplication rate pct 19.06 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 62/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 139 extrapolated ×1.2 100%

pct q30 bases 58.8 measured ×1 0%

duplication rate pct 19.06 measured ×0.5 65%

adapter content pct 0 measured ×0.4 100%

QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0