SRR1297160

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

57/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

WGS of Escherichia coli from obsolete ABI SOLiD platform with poor base quality (Q30=63%, scored zero) — a fundamental obstacle for variant calling. Low evidence_strength (0.61) reflects extrapolated rather than measured coverage. The combination of subthreshold Q30 and outdated sequencing technology make this dataset unfit for modern reuse.

Data type / assay

WGS

Organism

Escherichia coli

Instrument

AB 5500xl Genetic Analyzer

Platform

ABI_SOLID

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 853002400 reported

total reads 17060048 reported

mean coverage 185.4 extrapolated

n content pct 0 measured

pct q20 bases 83.4 measured

pct q30 bases 63 measured

gc content pct 50.2 measured

mean read length 50 measured

mean base quality 27 measured

adapter content pct 0 measured

duplication rate pct 28.75 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 57/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 185.4 extrapolated ×1.2 100%

pct q30 bases 63 measured ×1 0%

duplication rate pct 28.75 measured ×0.5 35%

adapter content pct 0 measured ×0.4 100%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0