Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR1299418

SRA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

51/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq from fly (Therinia lactucina) on HiSeq 2000 (100 bp). Grade F (51/100) with poor base quality as the driver: Q30 only 75.7% (scores 28/100) and mean quality 30.6 (scores 43/100) both fall well below standard. All metrics measured; the low base quality introduces systematic error in base calling and will significantly reduce accuracy for transcript quantification, limiting reuse.

Data type / assay
bulk-RNA-seq
Organism
Therinia lactucina
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1744659400 reported
total reads 17446594 reported
n content pct 0.003 measured
pct q20 bases 84.6 measured
pct q30 bases 75.7 measured
gc content pct 48.4 measured
mean read length 100 measured
mean base quality 30.6 measured
adapter content pct 0.31 measured
duplication rate pct 42.41 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 51/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.7 measured ×1 28%
mean base quality 30.6 measured ×0.6 43%
adapter content pct 0.31 measured ×0.4 100%
duplication rate pct 42.41 measured ×0.4 72%
QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0